Regeneration Potential via Somatic Embryogenesis in Cotton (Gossypium hirsutum L.)

Regeneration Potential via Somatic Embryogenesis in Cotton (Gossypium hirsutum L.)

Ahmad Al Jouma(1) P. Vindhiya Varman(2) T.S. Raveendran(2) P. Balasubramanian(2) and S. Mohan(2)

(1). Administration of Cotton Research, General commission for Scientific Agricultural Research, Damascus, Syria

(2).Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore, India.

(*Corresponding author Dr. Ahmad Al Jouma. Email: cottonresearch1@gmail.com).

Received: 05/09/2021                                 Accepted: 15/09/2021

Abstract

Agrobacterium–mediated transformation and regeneration via somatic embryogenesis remains the method of choice for regenerating of transgenic cotton plants. Cotyledon and hypocotyl explants of three cotton genotypes viz., MCU 12, Coker 201 and Coker 310 were cultured on different callus induction media. CIM2 medium containing 0.1 mg/l of 2, 4-D and 0.5 mg/l of kinetin recorded maximum callus induction frequency. Regeneration potential via somatic embryogenesis was found to be highly genotype dependent. Coker 310 and Coker 201 were able to produce somatic embryos, whereas MCU 12 did not evoke somatic embryogenesis. SEM8 medium containing an additional supplement of KNO3 (1.9 g/l) recorded the highest frequency of somatic embryogenesis irrespective of explant types used in Coker genotypes. However, cotyledon was more responsive to somatic embryogenesis than hypocotyls. Between the Coker genotypes, Coker 310 recorded higher frequency of embryogenic calli induction (92.7 %), somatic embryo induction (76.7 %) and somatic embryo maturation (43.0 %). The best regeneration response was observed on PRM3 medium containing 0.1 mg/l of GA3 and 1.0 mg/l of IAA. Coker 310 recorded the highest regeneration frequency of 42.7% with cotyledon explants. The complete protocol for successful regeneration of Coker genotypes through somatic embryogenesis was optimized in this study.

Key worlds: Cotton, Plant regeneration, Somatic embryogenesis, Coker genotypes.

Full Paper: PDF

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